Biotechnology Methods

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Manipulate and grow different microorganisms Describe the DNA structure and the flow of genetic information.

What is Biotechnology?

This includes DNA replication, gene transcription, and protein translation. Describe the roles of microorganisms in disease, environmental cycles, industrial applications, and biotechnology. Describe and perform molecular biology and genetic engineering techniques with particular emphasis on restriction digests, cloning, Southern blotting, Western blotting, PCR, and DNA typing.


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Describe the applications of molecular biology and genetic engineering techniques in human health and welfare, medicine, agriculture and the environment. Describe the use of the human genome project, gene therapy, molecular diagnostics, forensics, transgenic plants and animals, animal cloning, microorganisms in industrial biotechnology.

Describe in theory and the different genetic sequence databases Demonstrate the use of good laboratory procedures Describe issues related specifically to biotechnology industry, such as understanding the product development process, ethical, legal and social concerns Learning Outcomes By the completion of your biology courses at Oakton, you will have gained the experience to Think critically — identify, define, analyze, interpret, and evaluate ideas, concepts, information, problems, solutions, and consequences.

Department of Science, Technology, Engineering & Mathematics: Biotechnology

This includes the ability to compute and comprehend quantitative information and to engage in the scientific process. Communicate — communicate ideas, concepts, and information through written and oral means. Collaborate with people of diverse backgrounds and abilities. Demonstrate literacy — demonstrate the ability to read critically within content areas. Use technology to locate, evaluate, and communicate data, information, ideas, and concepts.

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Assess, critique, and select from a variety of information resources. Students and employees at Oakton Community College are required to demonstrate academic integrity and follow Oakton's Code of Academic Conduct.

There are serious consequences to violations of the academic integrity policy. Oakton's policies and procedures provide students a fair hearing if a complaint is made against you. If you are found to have violated the policy, the minimum penalty is failure on the assignment and, a disciplinary record will be established and kept on file in the office of the Vice President for Student Affairs for a period of 3 years.

Spectrophotometric analysis for maximum resolution 16 Gene Therapy and stem cell research lab reports and presentations Lab reports and presentations 17 FINAL week exam exam. Course may be taught as face-to-face, hybrid or online course. May vary depending on instructor, but may include: mandatory attendance during lectures active participation in class and satisfactory completion of written assignments satisfactory performance in written lecture tests or quizzes Course may be taught as face-to-face, hybrid or online course.

Note: Current textbook information for each course and section is available on Oakton's Schedule of Classes. All students are expected to fulfill essential course requirements.

The College will not waive any essential skill or requirement of a course or degree program. Oakton Community College is committed to maintaining a campus environment emphasizing the dignity and worth of all members of the community, and complies with all federal and state Title IX requirements. You can watch animations of PCR at these links:. The Polymerase Chain Reaction.

Biotechnology - Wikipedia

The polymerase chain reaction involves three steps. High temperatures are needed for the process to work. The enzyme Taq polymerase is used in step 3 because it can withstand high temperatures. Use this resource and the videos associated with this resource to answer the questions that follow. Biotechnology Methods Biotechnology is the use of technology to change the genetic makeup of living things for human purposes. Gene Cloning Gene cloning is the process of isolating and making copies of a gene.

In isolation, an enzyme called a restriction enzyme is used to break DNA at a specific base sequence. This is done to isolate a gene. A plasmid is circular DNA that is not part of a chromosome and can replicate independently.

Evaluation Copy

Ligation is illustrated in Figure below. In transformation , the recombinant DNA is inserted into a living cell, usually a bacterial cell. Changing an organism in this way is also called genetic engineering. Selection involves growing transformed bacteria to make sure they have the recombinant DNA. This is a necessary step because transformation is not always successful. Only bacteria that contain the recombinant DNA are selected for further use. Summary Biotechnology is the use of technology to change the genetic makeup of living things for human purposes.

Gene cloning is the process of isolating and making copies of a DNA segment such as a gene. The polymerase chain reaction makes many copies of a gene or other DNA segment. Explore More Use this resource and the videos associated with this resource to answer the questions that follow. Who developed PCR? What does PCR allow?


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  • Biotechnology;
  • Learning Outcomes.
  • Biotechnology Methods?

Describe the 3 steps involved in PCR.

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